Applications of Biosurfactant in Agriculture explores the use of beneficial microorganisms as an alternative to current synthetic plant protection strategies. The book highlights a range of renewable raw substrates including agro-industrial waste as a dependable and cost-effective technology for the mass production of biosurfactant, emphasizes the formulation of biosurfactants using a full-factorial design, scientometric assessment, and presents mathematical modeling for the enhancement of production processes. Recent biotechnological techniques such as functional metagenomics that could help in the molecular characterization of novel biosurfactant with multifunctional activities majorly from uncultured and unexploited microbes available in the soil biosphere are also explored. This book identifies possible modes of action by which nutrients are normally released to plants through the formation of metal-biosurfactant complexes and presents recent research findings on the utilization of biosurfactants for the management of mycotoxins and microorganisms when evaluated in the field and in greenhouses. Finally, the book emphasizes the application of biosurfactants as a form of potent antibiotics for the management of several zoonotic diseases and in animal husbandry.